多因素AD动物模型的建立及针刺对其学习记忆功能的影响

Establishment of Multi-factor AD Animal Model and Acupuncture Effect on Its Memory

为研究针刺对老年性痴呆 (AD)的疗效。 70只大鼠分成 7组 :正常对照组 (A)、模拟对照组 (B)、氯化铝 (AlCl3 )组 (C)、SCOP组 (D)、AlCl3 +SCOP组 (E)、针刺组 (F)、药物组 (G)。除A、B两组外 ,其余各组大鼠腹腔注射D 半乳糖 ,在此基础上 ,予以相应处理 :C组胃饲AlCl3 ,D组注射SCOP ,E组胃饲AlCl3 +注射SCOP ,F、G组以E组法造模并分别予针刺 (大椎、百会 )、喂服药物哈伯因 ,疗程 30d ,B组模拟E组 ,以生理盐水鼻饲和腹腔注射 ,A组不作任何处理。结果 :C、D、E 3组大鼠水迷宫游出时间、错误次数 ,跳台的触电潜伏期、3min错误次数 ,与B组比较均有改变 ,其中E组最为明显 ;F、G组的各项指标较E组改善 ,且两组间基本相同。提示 :多因素损伤能更好地突出AD的特征 ;针刺能提高AD动物的学习记忆功能。

To study the acupuncture effect on Alzheimer's disease(AD),seventy rats were randomized into 7 groups:group A(normal group),group B(control group),group C with AlCl 3,group D with ACOP,group E with AlCl 3 and ACOP,group F with acupuncture treatment and group G with medicine treatment.Except groups A and B,the rats in other groups were given abdominal injection of D-galactose and then treatment:group C with AlCl 3 by injection,group D with SCOP by perfusion,group E with AlCl 3 by perfusion and SCOP by injection,groups F and G modeled as group E and respectively treated by needling Dazhui,Baihui,and with Hupperzine A for 30 days;group B was treated with mormal saline by perfusion and abdominal injection,group A treated by nothing.Results:in comparison with group B,time taken to swim out of the water labyrinth,error frequency,electric latent period and 3-minute error frequency in groups C,D and E changed,especially in group E;the indexes in groups F and G improved more significantly than those in group E,but similar between group F and G.It is suggested that multi-factor injury can better reflect AD and acupuncture can improve the memory.