胰岛素纳米粒的制备

Preparation of insulin nanoparticles

目的 制备具有较高药物包封率的胰岛素纳米粒。方法 分别以海藻酸钠和壳聚糖为包裹材料 ,制备海藻酸钠纳米粒和壳聚糖纳米粒。用透析法测定胰岛素包封率 ;考查海藻酸钠浓度及固化剂量对形成海藻酸钠纳米粒大小、包封率的影响 ;研究不同配比多聚磷酸钠对壳聚糖形成纳米粒理化性质的影响。结果 经筛选得到形成海藻酸钠纳米粒的最佳固化剂氯化钙配比 ,测得粒径为 0 .10 μm(D50 ) ,选用合适浓度的多聚磷酸钠作为壳聚糖纳米粒的固化剂 ,测得粒径为 0 .13μm(D50 )。海藻酸钠纳米粒和壳聚糖纳米粒的胰岛素包封率分别达到 89.3%和 92 .1%。结论 选用海藻酸钠及壳聚糖制备胰岛素纳米粒 ,方法简便 。

OBJECTIVE: To prepare the insulin nanoparticles with high envelopment. METHODS: The insulin nanoparticles were prepared with sodium alginate or chitosan and the enveloped ratios of insulin were determined by dialysis. The effects on the characteristic of nanoparticles by the concentrations of sodium alginate and the quantities of solidified agent including calcium chloride or tripolyphosphate were selected. RESULTS: The sizes of the nanoparticles prepared at the optimized concentration of solidified agent were 0.10 μm (D50) and 0.13 μm (D50) and the enveloped ratios were 89.3% and 92.1%, respectively. CONCLUSION: It was easy to prepare the insulin nanoparticles with sodium alginate or chitosan, with a high envelopment.