重组人胸腺素α原体外对IFN-γ,IFN-α及TNF-α的影响

EFFECT OF RECOMBINANT PROTHYMOSIN α ON SECRETION OF IFN-γ, IFN-α AND TNF-α IN VITRO

目的 体外观察重组人胸腺素α原 (prothymosinα ,ProTα)对几种重要细胞因子分泌的影响。方法 用脾淋巴细胞、脾巨噬细胞及腹腔巨噬细胞 ,以ELISA法检测ProTα对IFN γ ,IFN α和TNF α分泌的影响。结果 1× 10 - 7mol·L- 1 ProTα明显促进脾细胞分泌IFN γ(P <0 0 5 ) ,该浓度的ProTα也明显刺激小鼠脾巨噬细胞分泌IFN α(P <0 0 1) ;在小鼠腹腔巨噬细胞中 ,ProTα能明显刺激IFN α和TNF α的分泌 (P <0 0 1)。结论 ProTα对细胞因子IFN γ ,IFN α和TNF α的分泌均有促进作用。

AIM To study the effect of prothymosin α (Pro Tα) as a fusion protein on secretion of IFN γ, IFN α and TNF α in vitro . METHODS The in vitro study was carried out on the culture of splenocytes,splenic and peritoneal macrophages isolated from Balb/c mice. Splenocytes were incubated with various concentrations of Pro Tα ( 1×10 -7 ～1×10 -10 mol·L -1 ) with or without Con A (5 μg·mL -1 ) for 72 h. Splenic and peritoneal macrophages were respectively treated with Pro Tα (1×10 -7 ～1×10 -10 mol·L -1 ) in the presence of LPS (10 μg·mL -1 ) for 24 h. Then IFN γ, IFN α and TNF α levels in the supernatant were detected by ELISA. RESULTS Pro Tα (1×10 -7 mol·L -1 ) was found to obviously increase IFN γ level ( P <0 05) in the supernatant of splenocytes compared with the control group. Moreover, Pro Tα (1×10 -7 mol·L -1 ) significantly induced the secretion of IFN α ( P <0 01) and TNF α ( P <0 01) in splenic and peritoneal macrophages. CONCLUSION In vitro , Pro Tα could increase the secretion of IFN γ , IFN α and TNF α .