肿瘤抗原MAGE-A家族成员进化关系的初步研究

Study on the evolutional reIationship among the member of MAGE-A family

目的：探讨肿瘤抗原MAGE-A家族成员间的同源性，发现公共CTL表位对抗肿瘤逃逸。方法：采用1999DNNAstar软件Windwow95/98平台支持下的蛋白质核酸序列编辑、分析工具对MAGE-A家族成员进行开放阅读框、蛋白及其CTL序列同源性分析井构建种系发生树。结果：表明MAGE-A家族核苷酸同源性高达57．6％-98.0％，种系发生树显示各成员来源于同一祖先井在不同时间分野，且部分CIL表位高度相似。结论：MAGE-A 家族成员来自于同一祖先的同一家族，在肿瘤细胞中表达存在异质性，故此研究结果将有利于在发展肿瘤治疗性细胞免疫疫苗中选择合适的CTL表位。

Abstract Objective:To explore homology in mage-A family and find some common epitopes recognized by cytotoxic T lymphocyte(CTL) to oppose tumor escape.Methods:The amino acid sequence,differents open reading frame(ORF) and the known CTL epitope of themember of MAGE-A family,were analyzed using DNAstar software, and phylogenetic tree is also constructed. Results: MAGE-A family shareidentity nucleotide 57.6% - 98.0%,the phylogenetic tree showed that they are derived from a common ancestor at different time, as well asfound some CTL epitopes high similarity. Conclusion: MAGE-A family come from common an ancestor, but they bave many differences in thepattern of expression in tumor.This study can help to find the best CTL epitope vaccine to cure tumor.