微囊化人胎胰岛移植治疗小鼠实验性糖尿病的观察

Xenograft of microencapsulated human pancreatic islets to diabetic mice

目的 :探讨微包囊技术在解决胰岛移植免疫排斥问题中的作用。方法 :将用链脲霉素 (STZ)制备的合格糖尿病模型鼠 2 1只随机分为 3组 ,每组 7只。空囊组腹腔内植入 5 0 0～ 6 0 0个空囊 ,游离胰岛组植入经胶原酶消化制备的人胎胰岛细胞 10 0 0± 10 0个 ,微囊组植入 10 0 0± 10 0个微囊包裹的胰岛细胞。结果 :游离胰岛组和微囊组小鼠在完全停用胰岛素的情况下 ,术后血糖分别降至 7.94± 2 .36mmol.L-1和 7.0 7± 1.15mmol.L-1,与空囊组比较差异有统计学意义 (t=13.170 P <0 .0 0 1,t=2 4 .999 P <0 .0 0 1) ,分别持续 7.4 3± 3.4 2天和 78.4± 2 1.2 7天 (t =8.6 5P <0 .0 0 1)。结论 :该微囊化人胎胰岛移植具有良好的组织相容性和免疫隔离作用 。

Objective:To observe the efficacy of microcapsules to prolong islet xenografts survival in mice.Methods:Human fetal pancreatic islets were isolated from the embryo which was obtained from legal abortion(gestational age 16～24 weeks) with collagenase and enclosed in semipermeable alginate BaCl 2 capsules.Diabetic BALB/ C mice induced with streptozotocin were divided into 3 groups.Each group had 7 mice.Then transplantation was performed.Results:Transplantation of 1000±100 encapsulated fetal islets into the peritoneal cavities of 7 BALB/ C mice restord normalglycemia for 78.4±21.27 days without immunosuppression.The second group of 7 diabetic mice received an equal number of uncultured pancreatic fragments.These unprotected xenografts were functional for only 7.43±3.42 days,but high mortality occured.There was significant differences between the two groups(P<0.001).The 7 mice that received only empty capsules showed continuous hyperglycemia and high motality.There was no spontaneous diabetic remission among these animals.Conclusion:These results demonstrate that the semipermeable membranes can protect the islets from immune injuries and transplants of microencapsulated islets can effectively prolong xenograft survival without immunosupression.