丹参对梗阻性黄疸肾损害保护作用的实验研究

Protective effect of Salvia Miltiorrhizae on the renal injury of rats with obstructive jaundice

目的 :探讨梗阻性黄疸 (梗黄 )肾损害及丹参保护作用机制。方法 :将 36只大鼠随机分为对照组、胆总管结扎组和丹参治疗组 ,以结扎胆总管方法建立梗黄肾损害鼠模型 ,丹参治疗组每日每只腹腔内注射 2g丹参注射液。 3组大鼠分别于术后第 7、14、2 1和 2 8天活杀 ,检测血清和肾组织中丙二醛 (MDA)及超氧化物歧化酶 (SOD)的变化 ,并观察肾功能和肾病理组织学改变。结果 :与非治疗组相比 ,丹参治疗组的血清及肾组织中SOD活性显著升高 ,而MDA则显著下降 (P <0 .0 5 ) ,肾功能和肾病理组织改变也较轻。结论 :氧自由基所引发的脂质过氧化作用是梗黄鼠肾损害发病过程中的重要因素之一 ,应用丹参可明显降低肾脏病理组织损害程度。

Objective:To investigate the mechanism of Salvia Miltiorrhizae(SM) on protecting the renal injury of rats with obstructive jaundice.Methods:The 36 rats were randomly divided into control group,obstructive jaundice group and SM treated group.The renal injury of rats model with obstructive jaundice was established by ligating common bile duct.In the SM treated group,SM (2.0g/ d) was given through abdominal cavity.Animals were killed at the 7th,14th,21st and 28th day after operation respectively.The malondialdehyde (MDA) and superoxide dismutase (SOD) activity content of serum and renal tissue were determined,and the changes of renal function and histopathology were observed.Results:Comparing with non treated group,the serum and renal tissue SOD activity were markedly increased,and the MDA were obviously decreased(P<0.05,for both).The changes of the renal function and histopathology were minor in SM treated group.Conclusion:Free radicals play an important role in the pathogenesis of renal lipid peroxidization injury in rats with obstructive jaundice.SM may remarkedly reduce the severity of renal histopathological damage.