复方紫草油对烧伤创面一氧化氮、内皮素、丙二醛含量的影响

INFLUENCE OF COMPOUND RADIX ARNEBIAE OIL ON THE CONTENT OF NO 、ET-1 AND MDA IN THE WOUND AFTER THERMAL INJURY

目的 :探讨复方紫草油对于烧伤创面早期影响的可能机制。方法 :建立大白鼠深Ⅱ度烫伤模型 ,实验组以复方紫草油外用包扎、对照组以 1%SDAg外用、空白对照组创面不用任何药物 ,分别测定伤前皮肤、伤后 2、4、8、12及 2 4h各组创面中的NO、ET - 1的含量以及伤前皮肤、伤后 4和 2 4h各组创面的MDA含量 ,并计算各组ET - 1/NO的比值。结果 :实验组的创面ET - 1、NO、MDA含量较对照组及空白对照组均降低 ,实验组的ET - 1/NO的比值较空白对照组和对照组为低 ,并且较早地趋向于伤前比值。结论 :复方紫草油可能通过调节创面的ET - 1、NO的含量 ,优化ET - 1/NO的比值 ,从而减少创面组织细胞膜的脂质过氧化损伤程度 ;复方紫草油在减轻创面组织脂质过氧化损害方面较磺胺嘧啶银可能有较好的作用。

Objective:To study the influence of compound radix arnebiae oil in the wound after thermal injury. Method:Eighty_six SD rats (250～300g) of both sexes were randomly divided into 4 groups.One group (n=6) was served as nonburned control and the content of nitric oxide (NO),endothelin-1(ET-1) and MDA in the back skin was measured after exposed to 37℃ water for 20 seconds,then the ET-1/NO was calculated.The other groups were exposed to 80℃ water for 20 seconds resulting in a deep-second degree burn involving about 20% surface area.In the 2 nd group (n=20),nothing was used to protect the wound and it served as blank control group.The 3 rd group was used the 1% SDAg to protect the wound and served as control group.The 4 th group was used the compound radix arnebiae oil to protect the wound and served as experimental group.After 2,4,8,12,24 hours,the content of NO,ET-1 was measured in the wound of the 2 nd ,3 rd ,4 th group.After 4,24h the content of MDA was measured in the wound of the 2 nd ,3 rd ,4 th group.In the 2 nd ,3 rd ,4 th groups,the ET-1/NO was calculated in the 2,4,8,12,24 hours after thermal injury. Results:The content of ET-1,NO in the 2 nd ,3 rd ,4 th group was higher than that in the 1 st group and the content of MDA in the 3 rd ,4 th was higher than that in the 1 st ,2 nd group.The ET-1/NO in the 4 th group was lower than that in the 2 nd group and 3 rd group.Conclusions:The content of NO,ET-1 and MDA in the wound can increase after thermal injury,and the compound radix arnebiae oil maybe regulate the content of ET-1 and NO and decrease the production of MDA in the wound after thermal injury.