涎腺肿瘤中增殖细胞核抗原,bcl-2,c-myc与细胞凋亡关系的研究

Proliferating cell nuclear antigen,bcl-2,c-myc and its relationship with apoptosis in salivary gland tumor

目的 :探讨涎腺肿瘤中细胞凋亡及其调控基因bcl- 2、c -myc及增殖细胞核抗原 (PCNA)间的关系。方法 :采用脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记 (TUNEL)技术 ,免疫组织化学染色和HE染色对 18例多形性腺瘤和 5 2例恶性涎腺肿瘤中细胞凋亡和PCNA、bcl- 2、c -myc蛋白表达进行观察和比较。结果 :恶性涎腺肿瘤的凋亡细胞指数和增殖细胞指数明显高于多形性腺瘤 (P <0 0 5 ) ;恶性涎腺肿瘤中bcl- 2和c-myc蛋白表达率和表达强度明显高于多形性腺瘤 (P <0 0 5 )。结论 :细胞凋亡和调控基因异常在涎腺肿瘤中可能起重要作用 ,在涎腺肿瘤中 ,bcl-

Objective:In order to assess proliferating cell nuclear antigen (PCNA),bcl-2,c-myc and its relationship with apoptosis.we investigate PCNA,bcl-2,c-myc and its relationship with apoptosis in salivary gland tumor.Mothods:Apoptosis was detected in histological sections by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling(TUNEL)technique,PCNA,bcl-2 and c-myc were detected by immunohistochemical staining in 18 cases of pleomorphic adenoma and 52 cases of malignant salivary gland tumor.Results:The apoptosis index and PCNA count were clearly higher in malignant salivary gland tumors than that in pleomorphic adenoma(P<0.05)?The positive rate and staining intensity of bcl-2 and c-myc oncoprotein in malignant salivary gland tumors were significantly higher than those observed in pleomorphic adenoma(P<0.05)?Conclusion:We conclude that the abnormal regulation of apoptosis may play an important role in salivary gland tumors;bcl-2 and c-myc oncoprotein may likely block apoptosis in salivary gland tumors?