针药结合对全脑缺血大鼠脑组织钙调素活性影响的实验研究

Influence of Acupuncture Combined with Medicine on Calmodulin Activity in Rat Ischemic Cerebral Region

目的 :观察针药结合对脑缺血大鼠脑组织内活性钙调素 (CaM)含量变化的影响。方法 :采用大鼠全脑缺血再灌注模型 ,用磷酸二脂酶法测定正常对照组、模型对照组、电针四肢井穴加药组、电针四肢井穴组、假手术对照组脑组织活性CaM的含量。结果 :造模后CaM显著升高 (P <0 0 5～ 0 0 0 1 ) ,经电针治疗及针药结合治疗后CaM显著下降 (P <0 0 0 1 ) ,但针药结合治疗与单纯电针治疗组之间降低CaM的作用无显著差异 (P >0 0 5)。结论 :电针、针药结合均可明显降低脑缺血再灌注大鼠脑组织中活性CaM的含量。

Objective: To observe the influence of acupuncture combined with medicine on the content of calmodulin(CaM) activity in rat ischemic cerebral region. Methods: The animal models of the cerebral ischemia with reperfusion were made by electric coagulation of bilateral vertebral arteries and occlusion of the ipsilateral common carotid artery. A total of 45 Wistar rats were randomly divided into five groups: ① normal control group (n=9), ② model control group(n=9),③ sham operation group (n=9), ④ electroacupuncture group[EA of'Shangyang'(LI 1), 'Zhongchong'(PC 9), 'Lidui' (ST 45) and 'Zuqiaoyin'(GB 44), 2～20 Hz, 20 min, [WT5BX]n=9], ⑤ EA + medicine group (Venoruton 90 mg/200 g body weight was given by intragastric perfusion an hour before the model was made, , ⑤ EA + medicine group (Venoruton 90 mg/200 g body weight was given by intragastric perfusion an hour before the model was made, n=9). CaM activity of the ischmic cerebral tissues was measured by using phosphodiesterase method. Results:When cerebral ischemia occurred, CaM activity of the ischemic cerebral region elevated obviously in comparison with normal control group and sham operation group (P<0.05～0.001). Following administration of EA and EA + medicine, active CaM contents were significantly lower than that of model group (P<0.001). There was no evident difference between EA and EA + medicine groups in CaM contents (P>0.05). Conclusion: Both EA and EA+ medicine can reduce ischemia reperfusion induced increase of active CaM level in the rat.