蜂针对佐剂性大鼠关节炎滑膜细胞因子基因表达的影响

Effect of Acupoint-apitherapy on Cytokine Gene Expression of Synovial Tissues in Adjuvant Arthritis Rats

目的 :检测IL 1 β、IL 6在类风湿性关节炎 (RA)的表达 ,探讨蜂针治疗RA的作用机理。方法 :将 40只大鼠随机分为四组 ,采用PCR微板核酸杂交 ELISA方法检测滑膜IL 1 β、IL 6的mRNA表达水平。结果 :蜂针组和激素组IL 1 β、IL 6的mRNA表达水平无显著性差异 ,而与模型组比较有显著性差异。结论 :RA早期IL 1 β、IL 6的mRNA表达水平是上调的 ,蜂针治疗后可以抑制其转录和表达 。

Objective: To study the effect of acupoint apitherapy on the expression of IL 1β mRNA and IL 6 mRNA and to analyze its mechanisms in treatment of rheumatoid arthritis (RA). Methods: A total of 40 rats were randomly and evenly divided into normal control group, model group, hormone (hydrocortisone) group and acupoint apitherapy group. RA model was established by injecting Freund's adjuvant (0.05 mL)into the right hind paw. For rats of hormone group, intraperitoneal injection of hydrocortisone (10 mg/2 mL) was performed. In acupoint apitherapy group, 'Shenshu'(BL 23), 'Zusanli'(ST 36), 'Mingmen'(GV 4) and 'Dazhui'(GV 14) were stimulated with bee needle pricking method. IL 1β mRNA and IL 6 mRNA expression of synovial tissues (right knee joint capsule) was detected using PCR microplate sandwich hybridization technique. Results:In comparison with normal control group, IL 1β mRNA and IL 6 mRNA expression of synovial tissues increased considerably in model group (P<0.01). There was no statistical difference between acupoint apitherapy group and hormone group in IL 1β mRNA and IL 6 mRNA expression, but the expressions in these two groups were significantly weaker than those of model group. Conclusion:Acupoint apitherapy could induce upregulation of IL 1β mRNA and IL 6 mRNA expression in RA rats , which may be responsible for apitherapy generated relief of symptoms and signs of RA.