醛固酮合酶基因多态性与原发性高血压关系的研究

Association Between Aldosterone Synthase Gene-344C/T Polymorphism and Essential Hypertension in Chinese Population

目的 :探讨醛固酮合酶CYP11B2基因 -344C/T多态性与原发性高血压的相关性。方法 :运用多聚酶链反应—限制性片段长度多态性分析 (PCR RFLP)了解醛固酮合酶CYP11B2基因 -344C/T基因型在原发性高血压患者 (n =10 8,原发性高血压组 )和正常血压患者 (n =14 6 ,对照组 )的分布情况。结果 :等位基因C、T在原发性高血压组和对照组的分布频率分别为 0 2 8,0 72和 0 36 ,0 6 4,基因频率分布符合Hardy Weinberg平衡 ,样本具有群体代表性 ,两组人群的基因型和等位基因频率无明显差异 (P >0 0 5 )。结论 :在中国人群中 ,醛固酮合酶CYP11B2基因

Objective:The present study examined the association between CYP11B2-344C/T polymorphism and essential hypertension in Chinese population. Methods:The distribution of-344C/T polymorphism in essential hypertensive(EH)patients and normotensive subjects was identified by a polymerase chain reaction-based restriction fragment length polymorphism protocol. Results:The allelic frequencies were 0.28 for the C allele and 0.72 for the T allele in normotensive subjects,0.36 for the C allele and 0.64 for the T allele in EH patients.The allelic frequencies were in Hardy-Weinberg equilibrium.No significant differences in the genotype distribution (p>0.05) and C allelic frequencies (p>0.05) were seen between EH patients and normotensive subjects. Conclusion:These results suggest that the-344C/T polymorphism of CYP11B2 is not significantly correlated with in Chinese population.