激光诱变青霉PT95原生质体选育类胡萝卜素高产菌株

SELECTION OF HIGH-YIELD CAROTENOID PRODUCING STRAIN BY LASER MUTAGENESIS OF PROTOPLAST OF PENICILLIUM SP. PT95

采用激光对青霉PT95菌株的原生质体进行了诱变处理,选育到一株菌核生物量和类胡萝卜素含量均有显著提高的突变菌株L05。与出发菌株相比,L05菌株的菌核生物量提高了98.6％,菌核中的类胡萝卜素含量提高了28.3％,在查氏平板上的类胡萝卜素产率提高了154.0％。所选育的L05菌株经3次传代培养,菌落没有发生扇形变异,菌核生物量和类胡萝卜素含量没有明显改变,说明该菌株具有良好的遗传稳定性。

A mutant strain L05 was screened from its parent strain Penicillium sp. PT95 by laser irradiation of protoplast. When LOS strain was incubated in Czapek' s agar plates for 20 d, both the sclerotia biomass and carotenoid content accumulated in sclerotia increased significantly compared with that of PT95 strain, and the increase rate reached respectively 98.6% and 28.3% . The carotenoid yield of L05 strain reached 381ug/plate, which was 2.54 times higher than that of PT95. The character of both sclerotia and carotenoid high productivity remained stable after three times of subculture. No sectored colony appeared during subculture.