摘要
利用根尖体细胞有丝分裂中期染色体GiemsaC 分带和荧光原位杂交从普通小麦 大赖草Lr.2、Lr.7异附加系辐射后代中选育出 2个纯合易位系 :(1)易位系NAU6 18(MS14 2 3) ,2n =4 4 ,易位染色体由大赖草Lr.7染色体的大部分 (约 5 / 6 ,包括着丝粒 )及小麦染色体 1A短臂的一部分 (近端约 1/ 3)组成 ,外源染色体片段的长度约占易位染色体总长度的 4 / 5 ;(2 )易位系NAU6 0 1(MS10 1 4 ) ,2n =4 2 ,易位染色体由小麦染色体 4B的整个短臂和 4B长臂近着丝粒部分 (1/ 3)及大赖草Lr.2短臂的绝大部分组成 ,外源染色体片段占易位染色体长臂的 1/ 2。对易位系进行的双端二体测交分析证实易位所涉及的小麦染色体分别为 1A和 4B。连续 3年单花滴注法进行的田间赤霉病抗性接种鉴定结果表明 ,普通小麦 大赖草异源易位系NAU6 18(MS14 2 3)对赤霉病的抗性与抗病对照品种苏麦 3号相仿 ,易位系NAU6 0 1(MS10 1 4 )对赤霉病抗性低于苏麦 3号 。
Two Triticum aestivum Leymus racemosus disomic translocation lines were selected and identified from irradiated progenies of T.aestivum L.racemosus addition lines Lr.2 and Lr.7 by somotic chromosome C banding and fluorescence in situ hybridization by using L.racemosus genomic DNA as probe. NAU618(MS142 3), 2n=44, is a disomic addition translocation line with a pair of translocated chromosome consisting of 5/6 length of \$L.racemosus\$ chromosome Lr.7 (including centromere) and 1/3 distal part of wheat chromosome 1A short arm. The alien segment is about 4/5 of the length of translocated chromosome. NAU601(MS101 4), 2n=42, is a disomic translocation lines with a pair of translocated chromosome consisting of 4B short arm, interstitial 1/3 part of 4BL and almost Lr.2S, the alien segment is a half of the long arm of translocated chromosome. The results of test cross analysis with T.aestivum c.v Chinese Spring double ditelosomic combine meiotic chromosomes C banding indicated that the translocated chromosomes in these two translocation lines were involved in chromosome 1A and 4B respectively. Their resistance to wheat scab was identified using single floret inoculation in three successive years and showed that the resistance of NAU618(MS142 3) was similar to that of resistant check variety Sumai 3, and NAU601(MS101 4) was lower than Sumai 3 but much higher than suscebtible parent Chinese Spring.
基金
国家自然科学基金 (3 9893 3 5 0 )
江苏省自然科学基金 (BK970 90 )
高校博士点基金 (970 2 0 5 )
美国Mcknight基金资助~~
