利用离果山羊草3C染色体诱导簇毛麦4V染色体结构变异

Structural Changes of 4V Chromosome of Haynaldia villosa Induced by Gametocidal Chromosome 3C of Aegilops triuncialis

通过普通小麦农林 2 6 离果山羊草 3C异附加系与普通小麦 簇毛麦 4V(4D)代换系杂交 ,杂交F1代与普通小麦回交 ,综合运用染色体构型分析、C 分带和荧光原位杂交等技术从BC1F2 、BC1F3 代中鉴定出涉及簇毛麦 4V染色体的易位系、端体、等臂染色体系等变异植株 ,表明离果山羊草 3C染色体可有效诱发簇毛麦 4V染色体结构变异 ,是创造小麦 簇毛麦

Chromosome 3C of Aegilops triuncialis was discovered with ability to be transferred preferentially in the case of its monosomic status in wheat background, whereas, those gametes without 3C would result in chromosome structural changes including deletions and translocations. In the present study, Triticum aestivum Haynaldia villosa substitution line 4V(4D)developed in our laboratory, was crossed to T.aestivum c.v. Norin 26 Aegilops triuncialis 3C addition line, and the hybrids F 1 were then backcrossed with common wheat in order to induce structural changes of 4V. Both chromosome C banding and genomic in situ hybridization was applied to search such chromosome variations. In this case, total genomic DNA of Haynaldia villosa was labelled by Biotin 11 dUTP as probes and total genomic DNA of Chinese Spring as the block. Moreover, several chromosome changes within common wheat such as isochromosome 1BL.1BL(B39 2) and others were also revealed. The result indicated that two translocation lines T4VL.3AS(A47 10 3)and T4VS.4DL(A47 25 4), two telocentric chromosome lines A47 7 2(4VS) and A47 32 2(4VL), and two isochromosomes including 4VS.4VS(A47 23)and 4VL.4VL(A412 5 4)were identified from BC 1F 2 or BC 1F 3.This result indicated that gametocidal chromosome 3C of Aegilop triuncialis could effectively induce structural changes of both chromosome 4V of Haynaldia villosa and chromosomes of wheat.