低浓度内皮素-1对活性氧抑制肺表面活性物质脂质合成的保护

Protective effect of low concentration endothelin-1 on the reactive oxygen-induced inhibition of pulmonary surfactant lipid synthesis

在离体肺组织培养模型上观察低浓度 (1～ 10 0pmol/L)内皮素 1(endothelin 1,ET 1)对活性氧所致肺表面活性物质 (pulmonarysurfactant,PS)脂质合成障碍及PS脂质主要组分磷脂酰胆碱合成限速酶CTP :磷酸胆碱二胞苷酰基转移酶 (phosphorylcholinecytidylyltransferase ,CCT)活性的影响。结果显示 :(1)黄嘌呤 黄嘌呤氧化酶超氧阴离子生成系统呈剂量依赖性地降低肺组织 3 H 胆碱的掺入量 ;(2 )ET 1可减轻活性氧所致 3 H 胆碱掺入量的减少和肺组织丙二醛含量的增高 ;但对肺组织超氧化物歧化酶、过氧化氢酶及总抗氧化能力无明显影响 ;(3)ET 1可分别提高和降低肺组织细胞微粒体和胞浆的CCT活性 ,并可减轻活性氧所致肺微粒体CCT活性的降低。结果表明 ,低浓度ET 1具有保护肺微粒体的CCT活性、减轻氧化性肺损伤所致PS合成障碍的作用 。

The effects of endothelin 1 (ET 1) at low concentration (1～100 pmol/L) on the reactive oxygen induced inhibition of both pulmonary surfactant (PS) lipid synthesis and the activity of CTP: phosphorylcholine cytidylyltransferase (CCT), a rate limiting enzyme in biosynthesis of phosphoatidylcholine (PC), were studied in cultured lung explants without serum. The xanthine xanthine oxidase superoxide anion generating system decreased 3H choline incorporation into PC in a dose dependent manner in cultured lung explants. ET 1 reduced both the reactive oxygen induced decrease in 3H choline incorporation and the increase in malondialdehyde (MDA) content of lung tissues, but did not change the levels of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and the total antioxidant capability in the lung explants. ET 1 enhanced microsomal CCT activity of the lung tissues, while it decreased cytosolic CCT activity of lung tissues. ET 1 also prevented the inhibitive effect of reactive oxygen on microsomal CCT activity in the lung explants. These results suggest that ET 1 at low concentration can protect the microsomal CCT activity and reduce the inhibition of PS lipid synthesis induced by oxidant lung injury. The protective mechanism of ET 1 is not relative to the pulmonary endogenous antioxidant defense system.