脂多糖通过诱导白细胞介素-1的生成引起迷走传入神经活动

The activation of vagus afferent in response to lipopolysaccharide: the role of interleukin-1

为探讨脂多糖 (lipopolysaccharide ,LPS)引起迷走传入神经活动是否可能通过白细胞介素 1(interleukin 1,IL 1)的作用 ,将Wistar大鼠随机分为LPS实验组和生理盐水对照组 ,用免疫组织化学方法检测迷走神经结状神经节c Fos及CD14的表达以及腹腔迷走神经周围Mac 1阳性巨噬细胞 (macrophage ,Mφ)。用L92 9细胞增殖法检测LPS刺激Mφ上清IL 1的生物活性。用原位杂交的方法检测迷走神经结状神经节Ⅰ型白细胞介素 1受体 (IL 1RⅠ )mRNA的表达。结果显示 ,LPS组迷走神经结状神经节神经元c Fos蛋白表达为阳性 ,而对照组迷走神经结状神经节神经元c Fos蛋白表达为阴性。LPS注射后 1h ,见腹腔迷走神经周围Mφ数量明显增多。Mφ在LPS刺激后 4 5min、1h和 2h时 ,IL 1生成明显增高。LPS组迷走神经结状神经节IL 1RⅠmRNA表达为阳性。以上结果提示 ,LPS引起迷走传入神经活动可能通过IL

To study the possibility of activation of vagus afferent in response to lipopolysaccharide (LPS) through interleukin 1 (IL 1), Wistar rats were randomly divided into LPS group and saline (NS) group The expression of c Fos, CD14 and Mac 1 were detected by immunohistochemistry staining IL 1 bioactivity was determined by L929 cell proliferation The expression of IL 1RⅠmRNA was detected by in situ hybridization Our results showed that there were some c Fos protein expression positive neurons in the nodose ganglion in LPS group, but no c Fos protein expression positive neurons in the nodose ganglion in control group The number of macrophages (Mφ) in the connective tissue surrounding the abdominal vagus increased significantly in response to LPS i p Forty five minutes after the application of LPS, the IL 1 bioactivity in the supernatant of Mφ was increased Positive IL 1RⅠmRNA neurons were also observed in the nodose ganglion in the LPS group The results indicate that vagus afferent is activated in response to LPS and that IL 1 production might be involved in the activation of vagus afferent