人血清载脂蛋白H放射免疫分析试剂盒的研制

Preparation of a Radioimmunoassay Kit for Human Serum Apolipoprotein H

目的 :从人血浆中分离纯化载脂蛋白H(apolipoproteinH ,apoH) ,经免疫家兔 ,获得特异性抗血清 ,采用Iodogen法进行12 5I标记apoH ,建立了人血清apoH放射免疫分析 (RIA)方法。结果 :方法灵敏度为2 33mg/L ,工作范围 5～ 32 0mg/L ,批内和批间CV分别为 4 93和 4 0 1% ,回收率为 95 7% ,与apo -AI、apo-B未见交叉反应。测定了 91例正常人血清apoH含量 ,正常值 :男性为 2 30 17± 5 6 4mg/L ,女性为 2 0 0 2 7± 4 2 5mg/L ,男女两组之间血清apoH水平差异无显著性。

Objective To establish a radioimmunoassay method (RIA) for the measurement of human serum apo-H. Methods Apolipoprotein H (apo-H) was isolated and purified from human plasma. The antisera against apo-H was obtained by immunizing rabbits, then, Apo-H was labelled with 125 I by iodogen method. Results The sensitivity was 2.33mg/L, and the assay range covered 5～320mg/L for apo-H. The intra-assay and inter-assay CV were 4.93% and 4.01% respectively, with a recovery rate of 95.7%. There was no cross reaction with apo-AI and apo-B. Normal mean value of serum apo-H was 230.17±5.64mg/L in males ( n=41 ) and 200.27±4.25mg/L in females ( n=50 ) with no significant sex difference.