转铁蛋白放射免疫分析方法的建立

Establishment of a method for RIA of Transferrin

目的 :在样品血清不作稀释的前提下建立转铁蛋白的放射免疫分析方法。方法 :通过降低标记抗原的比放射性 ,提高抗血清的浓度 ,改进曲线拟合方式等方法 ,扩大测定范围。结果 :抗血清亲和率为 3 82×10 -10 mol/L ,标记抗原的比放射性为 15 3mg/mCi,批间误差CV =5 38%批内误差CV =3 5 % ,曲线形态良好。 30例正常人的血清Tf含量为 2 81± 0 972mg/ml。结论 :在样品血清不作稀释的前提下建立转铁蛋白的放射免疫分析法 ,具有较好的临床实用价值。

Objective To develop a method for RIA of transferrin without dilution of the serun sample. Methods The measurable range was broadened through: Lowering of the specific radioactivity of the labelled antigen, increasing the concentration of the anti-serum and improve the curve fitting method.Results The antiserum affinity was 3.82×10 -10 mol/L, the specific radioactivity of the labelled antigen was 15.3mg/mci, inter-assay error CV=5.38%, intra-assay error CV=3.5% and the shape of the curve was good. For 30 normal control specimens, the meam TF content was 2.81±0.972mg/ml. Conclusion Development of a method for RIA of TF without previous dilution of the test serum was of clinical value.