铁蛋白标记抗原的高效液相色谱纯化

Re-purification of Labelled Ferritin Antigen With HPLC

目的 :通过高效液相色谱 (HPLC)对长期储存的铁蛋白抗原进行再纯化 ,提高铁蛋白标记抗原的质量。方法 :用高效液相色谱对铁蛋白抗原进行分析、纯化 ,用放射免疫分析法对纯化后的样品进行分析鉴定。结果 :经高效液相色谱分析 ,长期储存的铁蛋白抗原有明显的聚合现象 ,经高效液相色谱再纯化后 ,免疫活性及标记后的比放射性有明显改善。结论 :铁蛋白的高效液相色谱再纯化 ,对提高铁蛋白放射免疫分析试剂盒的质量有较大的意义。

Objective To improve the quality of long-term stored labelled ferritin antigen with HPLC. Methods The antigen was analyzed and purified with HPLC and again analyzed with RIA afterwards. Results Ferritin antigen underwent significant polymerization after long-term (angregation) storage. After repurification with HPLC, its immunoactivity and labelled specaific radioactivity were both significantly improved.Conclusion Quality of stored feritim RIA kit could be greatly improved after repurification with HPLC.