肺癌患者手术前后血清IL-8、IL-10和TGF-β_1水平的测定及临床意义

DETECTION AND CLINICAL SIGNIFICANCE ON THE LEVEL OF SERUM IL-8,IL-10 AND TGF-β_1 BEFORE AND AFTER OPERATION FOR LUNG CANCER

目的 :通过测定肺癌患者围手术期外周血清白细胞介素 - 8(interleukin- 8,IL- 8)、白细胞介素 - 10 (interleukin- 10 ,IL- 10 )和转化生长因子 - β1 (transform ing growth factor- β1 ,TGF- β1 )的水平 ,探讨其与肺癌的关系及临床意义。方法 :用酶联免疫吸附法 (EL ISA法 )测定了 32例肺癌患者手术前后及 2 0例健康人血清 IL- 8、IL- 10和 TGF- β1 水平。结果 :肺癌组术前 IL- 8、IL- 10和 TGF- β1 水平明显高于健康对照组 ,差异有显著性 (P<0 .0 1) ,且血清 IL- 8、IL- 10和 TGF- β1水平与肺癌的TNM分期有关 ,随肺癌的进展其水平逐步增高 (P<0 .0 1) ,与其病理分型无关 (P>0 .0 5 )。肺癌组术后其 IL- 8、IL- 10和 TGF- β1 水平较术前明显下降 (P<0 .0 1) ,但仍高于健康对照组 (P<0 .0 1)。结论 :肺癌患者存在免疫功能异常 ;肺癌切除术后 ,可以改善患者的免疫功能状态 ;动态观察 IL- 8、IL- 10和TGF- β1 水平将有助于肺癌的诊断及疗效评价 ,并可为肺癌的免疫治疗提供依据。

Objective:To study the mechanism of IL 8,IL 10,TGF β 1 in lung cancer′s pathogenesis by detecting serum IL 8, IL 10,TGF β 1 levels in patients with lung cancer.Methods:Serum IL 8, IL 10 and TGF β 1 levels were measured using an enzyme linked immunoadsorbent assay (ELISA) in 32 patients with lung cancer before surgery and after curative surgical resection and in 31 healthy volunteers.Results:The serum IL 8, IL 10 and TGF β 1 levels with lung cancer were significantly higher than those of the healthy volunteers ( P <0.01).Serum levels of IL 8, IL 10 and TGF β 1 were correlated significantly with tumor′s TNM stage ( P >0.01). And those were not associated with tumor's pathologic classification ( P >0.05).Serum levels of IL 8, IL 10 and TGF β 1 in patients with lung cancer after curative surgical resection of tumors decreased significantly comparing to those before surgery ( P <0.01), but were higher than those in healthy volunteers.Conclusion:The results implied that there were immune abnormalities in patients with lung cancer.It implies that preliminary detecting serum IL 8, IL 10 and TGF β 1 levels may be helpful to the diagnosis and treatment of lung cancer .