失血性休克及复苏后腓肠肌与肠组织损伤敏感性差异的研究

Study on difference of injury sensitivity of gastrocnemius muscle and intestine after hemorrhagic shock and resuscitation

目的 :探讨腓肠肌和肠组织对缺血、缺氧损伤敏感性的差异。方法 :采用 Wistar大鼠失血性休克及复苏模型 ,随机分为 6组 :假手术对照组 ,休克 90分钟组 ,休克 90分钟复苏 0 .5、1、2和 6小时组 ;进行腓肠肌和肠组织血流量和氧代谢的检测。结果 :失血性休克 90分钟 ,腓肠肌组织血流量降低程度重于肠组织 ,且复苏后血流量回升的速度慢于空肠、回肠和结肠 ;复苏后 2小时各组织氧耗和氧摄取率明显降低 ,肠组织降低程度重于腓肠肌。结论 :失血性休克及复苏后肠组织血流量降低程度虽轻于腓肠肌 ,但对氧的摄取和利用能力低于腓肠肌 ,这与肠组织对缺血。

Objective:To investigate the difference of sensitivity of tissue injury between gastrocnemius muscle and intestine during ischemia and hypoxia in the rat.Methods:Wistar rats were randomly divided into 6 groups:control group,group of hemorrhagic shock for 90 minutes,and groups of resuscitation for 0 5,1,2, 6 hours respectively after 90 minutes shock respectively.Blood flow and oxygen metabolism were assayed in gastrocnemius and intestine of rats.Results:The decrease of microcirculatory blood flow in gastrocnemius muscle was more critical than that of jejunum,ileum,and colon at 90 minutes after hemorrhagic shock and after resuscitation.At 2 hours resuscitation,oxygen consumption(O 2) and oxygen extraction rate(O 2ER) decreased significantly,and the decrease of these variables was more marked in the intestine than gastrocnemius muscle.Conclusions:Though the decrease in microcirculatory blood flow in intestine is lighter than that of gastrocnemius muscle,the ability of extracting and utilizing oxygen is more lower after resuscitation.Intestine is more sensitive to ischemia and hypoxia.