内毒素致伤大鼠白介素-10表达及肺组织激活蛋白-1活性的变化

Changes in pulmonary interleukin-10 mRNA expression and activator protein-1 activity in rats with acute lung injury induced by lipopolysaccharide

目的 :观察内毒素 (L PS)致伤大鼠血浆 IL 10及肺组织 IL 10 m RNA含量和激活蛋白 1(AP 1)活性的变化 ,探讨 AP 1对 IL 10基因表达的作用。方法 :采用 L PS(2、4、6和 8mg/kg)致伤 Wistar大鼠 ,在各时间点 (1、2、4和 6小时 )制备动脉血浆并取肺组织。采用酶联免疫吸附法 (EL ISA)检测血浆 IL 10含量 ,采用逆转录聚合酶链反应 (RT PCR)检测 IL 10 m RNA含量 ;采用凝胶迁移率分析法 (EMSA)检测 AP 1活性。结果 :1L PS可使血浆 IL 10含量及肺组织的 IL 10 m RNA含量和 AP 1活性同步升高 ;2 L PS≥6 mg/kg时 ,血浆 IL 10含量及肺组织的 IL 10 m RNA含量和 AP 1活性的高峰增长幅度显著加大。结论 :急性肺损伤或急性呼吸窘迫综合征时 IL 10基因表达增强与 AP 1活性同步升高有关。

Objective:To explore the relationship between interleukin10 (IL10)mRNA expression and activator protein1(AP1) activity in lung tissue in rats with acute lung injury(ALI) induced by lipopolysaccharide(LPS).Methods:Wistar rats were injured with increased doses of LPS(2,4,6,8 mg/kg) to cause the systemic inflammatory response syndrome(SIRS) or acute lung injury(ALI).Enzymelinked immunosorbent assay(ELISA),reverse transcriptasepolymerase chain reaction (RTPCR) and electrophoretic mobility shift assays(EMSAs) were respectively employed to measure plasma IL10 level,IL10 mRNA and AP1 binding activity in lung tissue in rats at 1,2,4,and 6 hours after LPS injection.Results:LPS challenge resulted in increased IL10 mRNA level and AP1 activity in lung tissue in rats,and the peak values of IL10 mRNA and AP1 activity were significantly elevated under LPS≥6 mg/kg .Conclusions:The obvious increase of transcription of IL10 gene accompanied by AP1 activity in lung tissue might be involved in the development of ALI/SIRS induced by LPS.