烧伤后大鼠肝脏的病理形态学变化与肝功能的关系

The relationship between liver function and pathological changes in burned rats

目的 :研究大鼠烧伤后肝脏早期损伤的病理组织学和超微结构的特点及其与肝功能的关系。方法 :采用 30 %总体表面积 度凝固汽油烧伤实验动物模型 ,用光镜和电镜动态观察致伤后 1、3、6、12、2 4和 48小时肝组织病理变化 ,并检测血清丙氨酸转氨酶 (AL T)、天冬氨酸转氨酶 (AST)含量。结果 :烧伤后 2小时 ,病理形态学显示 :肝细胞、肝窦内皮细胞有不同程度损害 ,伴肝窦内有较多中性粒细胞滞留 ,汇管区有较多炎细胞浸润 ,枯否细胞增生及吞噬增强。 AL T在伤后 1小时即有显著升高 ,12小时达峰值水平 ,AST变化与 AL T相似。结论 :肝脏是烧伤早期易受损伤的器官之一 ,早期肝脏的病理损伤可加重肝功能障碍。

Objective:To investigate the early pathological changes in liver after burns,and their relationship with the liver function in order to provide theoretical basis for clinical therapy.Methods:Rats were subjected to a 30% total body surface area(TBSA) full thickness thermal injury.The histological and ultrastructural changes in the liver,and serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were dynamically examined at 1,3,6,12,24,and 48 hours after burn injury.Results:In burn group,different morphological disorders of hepatocytes,and hepatic sinusoid endothelial cells,with polymorphonuclear sequestration were observed.The level of serum ALT was significantly increased at 1 hour after injury and reached peak level at 12 hours postburn.The change of serum AST in serum was similar to ALT.Conclusions:The results suggest that the liver is one of the susceptible organs following burn injury.To protect hepatocytes from injury is beneficial in improving the liver function.