中期因子mRNA在原发性肝癌中的表达及其意义

Increased midkine mRNA expression in hepatocellular carcinoma

目的 探讨中期因子 (MK)mRNA在原发性肝细胞癌 (HCC)中的表达及其意义。方法 收集 33例人肝细胞癌组织、配对癌旁肝组织及 1 0例肝良性肿瘤组织、5例正常肝组织 ,采用TRIzol一步抽提法提取总RNA ,应用逆转录 聚合酶链反应 (RT PCR)检测MKmRNA的表达。结果 2 8例肝细胞癌可见MKmRNA表达 ,阳性率为 84.8% ,而配对的癌旁组织中仅见 3例表达 ,正常肝脏和肝脏良性肿瘤组织无 1例阳性。统计学分析表明 ,肝细胞癌MKmRNA表达率与其他各组的差异有非常显著性 (P <0 .0 1 ) ,但肝细胞癌MKmRNA表达与其组织学类型、病理分级、瘤体大小、包膜状况及其甲胎蛋白 (AFP)值之间差异无显著性 (P >0 .0 5)。结论 肝细胞癌在mR NA水平上表达MK增加 ,检测MK表达情况可作为HCC的辅助诊断指标之一 。

Objective To investigate the expression of midkine (MK) mRNA in hepatocellular carcinoma (PHCC) and its clinical implications.Methods Liver tissues were obtained from 33 patients with HCC,10 with benign liver tumors and 5 with normal controls.Total RNA was extracted from tissues by using TRIzol method.MK mRNA expression was detected by using RT PCR.Results MK mRNA expression was detectable in the concerous tissues of 28 HCC patients (84.8%) and in the tissues adjacent to the concers of 3 cases.All the benign liver tumors and normal tissues were negative for MK mRNA expression (P<0.01).No significant difference was found in clinical and pathological parameters between MK mRNA negative and positive cases.Conclusion MK is expressed at mRNA level in the majority of HCC tissues and rarely in the adjacent tissues.MK might be used as a marker for HCC.