门静脉高压症鼠断流术前后食管中碱性成纤维细胞生长因子的表达

The expression of basic fibroblast growth factor in esophagus before and after devascularization in portal hypertensive rats

目的 探讨碱性成纤维细胞生长因子 (bFGF)在门静脉高压症断流术前后食管中的表达和意义。方法 雄性SD大鼠用门静脉缩窄术制备肝前型门静脉高压症 (PHPH)模型 ,以假手术 (SO)作为对照组 ,两组均取一部分在造模后 7～ 2 8d取 4个时间点观察 ,其余部分在造模后 3周行联合断流术 ,在术后 1～ 90d取 7个时间点观察 ;免疫组织化学法检测bFGF在食管中的表达。结果 PHPH组较SO组bFGF表达显著增高 ,差异有显著性 (P <0 .0 1 ) ,在断流术后两组均出现了bFGF表达高峰 ,但PHPH组表达时相较SO组显著延长 ,差异有显著性 (P <0 .0 1 )。

Objective To study the expression of basic fibroblast growth factor (bFGF) expression in esophagus before and after devascularization in portal hypertensive rats and its implication.Methods Prehepatic portal hypertension (PHPH) model in rats was induced by portal vein stenosis,and the shamoperated (SO) rats served as control group.After 3 weeks,all the rats were devascularized.Each of the two groups was checked up according to the stages after the two operations:1,2,3 weeks and 1 month after the portal vein stenosis (or sham operation),and 1,3,5 days,1,2 weeks and 1,3 months after devascularization respectively.The expression of bFGF was detected by the method of immunohistochemistry.Results The expression level of bFGF in PHPH rats was significantly higher than in SO rats (P<0.01).After devascularization,both two groups showed the peak of bFGF experssion,but the time of bFGF expression in PHPH rats was notably longer than in SO rats (P<0.01).Conclusion The increasing expression of bFGF may take part in the formation of esophagueal varices before and after devascularization in portal hypertension.