苯乙酸和二甲基甲酰胺对胶质瘤同源盒基因表达影响的比较

The effects of phenylacetate and dimethylformamide on homeobox gene expression in glioma cell line C_6

目的 观察苯乙酸 (PA)和二甲基甲酰胺 (DMF)诱导分化胶质瘤细胞C6 过程中同源盒(Hox)基因表达的变化。方法 用逆转录 聚合酶链反应 (RT PCR)及图像分析法 ,检测PA、DMF对胶质瘤细胞C6 诱导分化过程中Hox基因组P1、P2、P3及特异Hox基因的表达。Hox基因(组 )表达水平用基因 (组 ) / β 肌动蛋白 (β actin)灰度比值表示。 结果 Hox基因组P2在胶质瘤细胞C6 中表达明显低于P1、P3组 [0 .682 5± 0 .0 987<0 .881 7± 0 .0 73 1 ,0 .860 8± 0 .0 881 ,(P<0 .0 0 1 ) ]。应用PA后 ,P2组HoxB2基因表达明显上调 [0 .776 3± 0 .1 2 4 1 >0 .483 9± 0 .1 34 3 ,(P <0 .0 0 1 ) ] ;应用DMF后 ,HoxB2基因表达无显著变化 ,差异无显著性 (P >0 .0 5)。HoxB4基因在应用PA和DMF前后均未见表达。结论 PA对HoxB2基因mRNA水平的表达有明显的上调作用 。

Objective To observe the effects of phenylacetate (PA) and dimethylformamide (DMF) on Homeobox (Hox) gene expression in the process of inducing and differentiating glioma cell line C 6.Methods Using semiquantitative reverse transcription polymerase chain reaction (RT PCR) the expression of Hox genes mRNA in rat C 6 glioma cell line following differentiation induced by PA and DMF was detected.The level of Hox gene expression was expressed as ratio expression rate (RER) of Hox gene/β actin and the difference between C 6 cells and PA treated C 6 cells was analyzed by student’t test.Results Hox genes matching to primers P2 were weakly expressed in C 6 cells and the expression of HoxB2 mRNA was significantly up regulated in PA treated C 6 cells (P<0.001).DMF had no effect on expression of Hox gene.Conclusion It can be inferred from these findings that PA can up regulate the expression of Hox genes,but DMF has no effect on the Hox genes expression.