小肠结肠炎耶尔森氏菌血清群的研究——Ⅱ.我国小肠结肠炎耶尔森氏菌分群菌株的筛选和参考菌株的建立

STUDIES ON THE SEROGROUP OF YERSINIA ENTEROCOLITICA Ⅱ. SELECTION AND ESTABLISHMENT ON SEROGROUP REFERENCE STRAINS OF YERSINIA ENTEROCOLITICA

从我国收集到新分离的小肠结肠炎耶尔森氏菌1120株,经检定符合本菌特征的747株(占66.7%),并筛选出其形态、培养特征及生化特性典型的有335株,分成36个不同血清群,并分别找出各群代表株制出抗血清。试验结果证明,用本套菌株制备的群血清,灵敏性和特异性效价都优于国外引进的参考菌株制备的抗血清。通过实际使用,对国内新分离菌株检定能提高定群率2—3%。

Since 1980, we have collected 1120 strains of Yersinia enterocolitica, from the different parts of China. These strains have been obtained from various sources in man, animals and natural environment accompanied by their clinical or ecological information of Yersinia enterocolitica. The results of our tests have shown that the 747 strains have exhibited the clinical morphological and biochemical characteristies of Yersinia enterocolitica. Through compareing under the same conditions, out of the 747 strains 335 have been selected out with better antigenicity and have been produced antisera from their representative strains. This set of antisera is very satisfactory for its potency and specificity.This set of antisera is ready to supply and have good efficacy and application facilitated for control strains on identifying strains and their epiderniologic observation.