摘要
将含有大尺蠖核型多角体病毒(BsNPV)多角体蛋白基因(ocu)的BamHI-H片段克隆到表达载体pDR 540的BamHI位点,得到能抗高浓度氨苄青霉素(200μg/ml)的两个阳性重组体,其胞外总蛋白比亲本质粒高1.1—1.4倍。在大肠杆菌细胞中,BsNPV ocu基因在原核杂合启动子tac驱动下能高水平的表达,表达量达到0.8和4.7mg/ml。免疫沉淀反应证明,表达蛋白为BsNPV的包涵体蛋白,凝胶过滤法测定的平均分子量为32.1×10~3道尔顿。
The BamHI-H fragment containing polyhedrin gene (ocu) of Buzura suppressuria nuclear polyhedrosis virus (BsNPV) was inserted into BamHI site in plasmid pDR540 and two Apr positive recombinants were obtained. Total proteins Produced by clones are more 1.1—1.4 folds than that by parent strain carried plasmid pDR540 in the medium. Expression of ocu protein was 0.8 mg/ml and 0.47 mg/ml. The expression protein was isolated and purified by PAG electrophoresis and gel chromatograph. The molecular weight is 32.1×10~3 dalton.
出处
《微生物学报》
CAS
CSCD
北大核心
1991年第1期25-31,共7页
Acta Microbiologica Sinica
基金
国家科学基金
国家教委基金
关键词
昆虫病毒
BsNPV
ocu
表达蛋白
Buzura suppressuria nuclear polyhedrosis virus
Polyhedrin gene
Expression protein
