立克次体脂肪酸图谱及其相似性判别

GAS CHROMATOGRAMS OF FATTY ACIDS FROM RICKETTSIA AND THEIR SIMILARITIES

用气相色谱-质谱法分析了7株立克次体浓盐乙醚纯化物的脂肪酸成分,即R.prowazekii E株、R.conorii Simko株、R.rickettsii R株、R.sibirica Barbash株和246株、R.sinkiangensis Jinghe株以及R.heilungkiangensis 54株。所得脂肪酸色谱图中有近50个色谱峰,初步确认有以下16种:C_(11∶0)、2OH-C_(10∶0)、C_(12∶0)、2OH-C_(12∶0)、C_(13∶0)、C_(14∶0)、C_(15∶0)、3OH-C_(14∶0)、C_(16∶1)、C_(16∶0)、C_(17∶0)、C_(18∶2)、C_(18∶1)、C_(18∶0)、C_(19∶0)和C_(22∶0)。其中主要成分是直链饱和脂肪酸C_(16∶0)、C_(18∶0)及C_(14∶0)与不饱和脂肪酸C_(18∶1)、C_(18∶2)及C_(16∶1)。实验菌株脂肪酸图谱经改进的Kulik-Vincent相似系数法处理后,精河株和246株的相似系数为97.0%,54株和其他菌株的相似系数在81.6—94.6%之间。

Fatty acid compositions of 7 strains of Rickettsia were analyzed by a on-line GC/MS system. These strains were R. prowazekii E, R. conorii Simko, R. rickettsii R, R. sibirica Barbash and 246, R. sinkiangensis Jinghe, and R. heilungkiangensis 54. The samples were purified by means of the concentrated salt-ether method. There were about 50 peaks in the fatty acid profiles, and 16 of these peaks were determined, i.e. C_(22:0), C_(19:0), C_(18:0), C_(18:1),C_(18:2), C_(17:0), C_(16:0),C_(16:1), 3-OH-C_(14:0), C_(15:0), C_(14:0), C_(13:0), 2-OH-C_(12:0),C_(12:0), 2-OH-C_(10:0),and C_(11:0). The majar fatty acids were the saturated straight chain acids (e. g. C_(16:0), C_(18:0), C_(14:0)) and the unsaturated straight chain acids (e.g. C_(18:1),C_(18:2),C_(16:1)).Similarities of fatty acid profiles of tested strains were discriminated by the improved Kulik-Vincent method. The result showed that the KV's coefficient of strains Jinghe and 246 was 97.0%, and the KV', coefficient of strains 54 and the others was 81.6—94.6%.