三株梭菌通过生物转化形成熊去氧胆酸的研究

STUDY ON URSODEOXYCHOLIC ACID BIOTRANSFORMATION BY THREE STRAINS OF CLOSTRIDIA

用改进的薄层层析法定量测定了三株厌氧梭菌——产气荚膜梭菌(Clostridium perfringens)HS-10、丁酸梭菌(C.butyrium)DL-20和LQ-29形成熊去氧胆酸(UDCA)的生物转化能力,并用正交法确定了HS-10菌株的最佳转化条件。发现该菌株在含0.2mmol/L鹅去氧胆酸(CDCA)的RCM培养基中培养6—48小时内,UDCA转化率均在80%以上。而且,当CDCA的浓度高达0.8—1.0mmol/L时,其转化率仍在70%以上。此外,还初步发现未加任何营养成分的豆腐废水也可作为良好的转化培养基。本文是这两种菌能单独将CDCA转化为UDCA的首次报道。

Using the improved TLC procedure, we measured the conversion rates of ursodeoxycholic acid(UDCA) from chenodeoxycholic acid (CDCA) by 3 strains of clostridia——C. perfringens HS-10, C. butyricum DL-20 and LQ-29 isolated. The orthogonal test was used to determine the optical conditions of biotransformation for HS-10. It was found that the conversion rate of UDCA by HS-10 was over 80% during 6—48 h in RCM medium containing 0.2 mmol/L CDCA. The UDCA conversion rates were still over 70% when the CDCA concentrations were as high as 0.8—1.0 mmol/L. In addition, the WWBCP (Waste water from bean curd production) without any supplemental nutrients was preliminarily proved to be a potential inexpensive conversion medium.