假单胞菌DM11菌株的二氯甲烷脱卤素酶研究

STUDIES ON DICHLOROMETHANE DEHALOGENASE OF PSEUDOMONAS SP.STRAIN DM11

二氯甲烷(CH_2Cl_2)被广泛地用作清除剂、溶剂提取剂、冷冻剂和熏蒸剂,是一种主要的环境污染物。1980年 Rittmann 等首次从工业废水中分离出以二氯甲烷为唯一碳源和能源的细菌。

Dichloromethane dehalogenase wasisolated and purified from Pseudomonassp.strain DM11 by improved methods.The optimum reaction conditions for theenzyme are 100mmol/L KH_2PO_4(pH8.2),1mmol/L GSH,2mmol/L CH_2Cl_2 and30℃.The specific activities of theenzyme are 77.8,123.3,78.8,56.1 and60.3mkat/kg protein for CH_2Cl_2,CH_2Br_2,CH_2I_2,CH_2ClBr and CH_2ClI used as asubstrate,respectively.