深部真菌感染的微生物学诊断与防治研究

Study of Microbiology Diagnosis and Prophylactic Treatment on the Diseases of Deep-Fungi

用简单、快速的念珠菌分类鉴定法对657例不同患者标本进行了鉴定,结果121例分得真菌,阳性率18.24％。分得真菌153株,其中酵母样真菌97株,占63.4％;曲霉23株,占15.03％.酵母样真菌97株中念珠菌83株,占酵母样真菌的85.57％。念珠菌中又以白色念珠菌为主,其次为热带念珠菌、克柔氏念珠菌。深部真菌感染∞％以上是由念珠菌引起的。用ELISA和GC法检测念珠菌感染患者血清中Mn—Ag,结果表明患者血清中Mn—Ag浓度明显高于正常人,用念珠菌感染动物后24h,在血清中可测到Mn—Ag,是一个早期快速、特异诊断念珠菌血行播散的方法。丁香、黄连、桂皮、木香对真菌有抑杀菌作用。真菌感染与应用抗生素、激素的数量及时间密切相关。

657 specimens were examined, among which 121 cases(18.94％) were fungus-positive. 153 strains of fungi were obtained, of which 97 strains were yeast-like Candida(63.4％), 23 strains were Aspergillus(15.03％). And in the 97 strains of yeast-like Candida 83 strains were Candida(85.57％). Of the Candida, C albicans are the most common fungus and then in sequence the C tropicalis, C krusei. In deep fungus infections, more than 50％ are caused by Candida. In this paper, ELISA and CG methods are established to determine the Mn-Ag both in normal and patients'serum. The results showed that the amount of Mn-Ag in healthy and patients'sera was different. We observed that Mn-Ag can be determined 24 hours after Candida infection. The results of ELISA and CG are same. This indicates determination of Mn-Ag in serum is a sensitive rapid and specific method for diagnosising blood-speed Candida infections. In vitro test showed that lilac, berberis, vladimirda, cinnamomum cassia have higher activity for inhibiting fungi growth.