健康大鼠完全液体通气时血气、血压及心率的观察

Evaluation of Gas Exchange and Circulation during Total Liquid Ventilation in Health Rats

目的:探讨国产全氟碳化合物(PFC)作为液态呼吸介质用于完全液体通气(TLV)的可行性。方法:健康大鼠20只随机分为两组(1)PFC组(10只),用国产全氟萘烷(FDC)作为液态呼吸介质进行TLV。(2)生理盐水组:用生理盐水(NS)进行TLV。分别观察动物的存活时间,TLV前及TLV后5、15、30、45分钟(min)的动脉血氧分压(PaO_2)、二氧化碳分压(PaCO_2)、pH值、心率(HR)以及动脉平均压(MAP)的变化。结果:1.PFC组动物存活(49.1±8.4)min,NS组动物存活(2.5±3.4)min。2.PFC组TLV 5min时PaO_2均较基础状态(TLV前)明显降低,15、30、45min各时间点PaO_2较5min明显增高,15、30、45min PaO_2间无显著差异,TLV后PaCO_2进行性升高、pH进行性下降。5、15min时HR较TLV前无明显变化,30min起HR进行性下降。5min时MAP较TLV前无明显变化,15min起MAP进行性下降。结论:FDC介导的TLV能支持健康大鼠短期存活,可使PaO_2保持在8kPa以上。

Objective: To evaluate perfluorocarhon ( PFC made by China ) as liquid breath medium used in total liquid ventilation (TLV) . Methods: 20 health rats were randomized to undergo total liquid ventilation with PFC(n = 10) or normal saline (NS) (n = 10) . Rat survive time, heart rates (HR), mean arterial pressure (MAP), PaO2 , PaCO2 and pH were observed at 0 (before TLV), 5, 15, 30, 45 minutes after TLV.Results: 1.The animal survive time of PFC group is (49.1 ± 8.4) minutes , NS group (2.5 ± 3.4)minutes. 2. In PFC group, PaO2 of 5 minutes was significantly lower compared with 0 minutes, PaO2 of 15, 30 and 45 minutes was significantly higher compared with 5 minutes, there were no different of PaO2 between 15,30 and 45 minutes. PaCO2 was gradually increased and pH gradually decreased after TLV in PFC group. No different of HR between 5,15 and 0 minutes, HR of 30 and 45 minutes gradually decreased compared with HR of 15 minutes. MAP of 5 minutes was found no different compared with 0 minutes, MAP of 15, 30 and 45 minutes gradually decreased compared with MAP of 5 minutes. Conclusion: TLV with PFC can support health rats survive in short time and can keep PaO2 above 8 kPa.