检测外周血中p53基因突变诊断肺癌微转移的临床意义

Detection of p53 gene mutation in peripheral blood in the diagnosis of patients with lung cancer

目的 探讨检测肺癌患者外周血中p53基因突变的诊断意义。方法 应用PCR SSCP方法 ,对2 8例肺癌患者、8例良性肺疾病患者和 1 0例健康人外周血中的p53基因突变进行检测。结果 在 2 8例肺癌患者外周血标本中 ,4例检测出p53基因突变 ,阳性率为 1 4 .2 9%。其中鳞癌 1例 ,腺癌 1例 ,小细胞癌 2例 ;Ⅱ期患者 1例 ,Ⅲ期 1例 ,Ⅳ期 2例。 8例良性肺疾病患者和 1 0例健康人的外周血均未检测出p53基因突变。结论 检测外周血中p53基因突变有助于肺癌的诊断和肺癌病理分期。

Objective To study the diagnostic significance of detecting p53 gene mutation in peripheral blood of lung cancer patients. Methods PCR SSCP was used to detect p53 gene mutation in peripheral blood of 46 samples (lung cancer 28, benign lung diseases 8, healthy people 10). Results Of the 28 lung cancer patients, 4 cases (squamous cell carcinoma 1, adenocarcinoma 1, small cell lung cancer 2) had p53 gene mutation. The positive rate was 14.29%. Of the 4 positive lung cancer patients, one patient was in stage Ⅱ, one in stage Ⅲ, and the other two in stage Ⅳ. No p53 mutation was found in peripheral blood of 8 benign lung disease patients and 10 healthy persons. Conclusion Detection of p53 gene mutation in peripheral blood cells may be helpful to the diagnosis of lung cancer, especially to the accurate staging of lung cancer.