脑缺血再灌注对大鼠不同组织细胞凋亡及凋亡调控基因表达的影响

Effects of cerebral ischemia and rep erfusion injury on apoptosis of cell and expression of apoptosis gene in the different tissues of rats

目的探讨脑缺血再灌注(CI/RP)对大鼠不同组织细胞凋亡及凋亡调控基因表达的影响。方法应用TUNEL法和免疫组化法,分别测定CI/RP损伤后各组大鼠脑皮质、丘脑和胸腺组织细胞凋亡及凋亡调控基因bcl-2和Bax蛋白表达的变化。结果CI/RP损伤后大鼠脑皮质、丘脑和胸腺组织细胞凋亡率明显升高,凋亡调控基因bcl-2和Bax蛋白表达率也呈不同程度的升高,与对照组比较,差异非常显著(P<0.01)。电镜观察结果与之相似。结论CI/RP损伤后上述3种组织细胞凋亡率增高,凋亡调控基因bcl-2和Bax表达异常。该结果为临床早期防治CI/RP损伤提供了实验依据。

Objective To explore the effects of cerebral is chemia and reperfusion(CI /RP)on the apoptosis of cell and expression of apoptosis gene in the different tissues of rats.Methods The number of apoptosis cell and the e xpression of apoptosis gene bcl-2an d Bax proteins in brain cortex,thalamus a nd thymus in the various group of rats were respectively detected by the use of a terminal deoxynucleotidyl transferase mediated dUTP-biotin n ick end labeling(TUNEL)and immunohistochemical methods.Result The number of apoptosis cell in the three kind of tissues of rats after CI /RP injury was higher than those of th e control group(P<0.01),and so were the expressions of bcl-2and Bax apoptosis gene(P<0.01).Conclusion This study provides experimental basis for the prevention and treatment of CI /RP injury.