单胺氧化酶活性分析在HBV感染者中的应用

Analysis of Monoamine Oxidase Activity and Its Application in the Patients with Hepatitis B Virus

目的分析乙肝病毒(HBV)感染者血清单胺氧化酶活性,研究其临床应用价值.方法应用终点比色法检测血清单胺氧化酶(MAO)活性;ELISA法检测乙肝病毒标志物;PCR法检测HBv DNA;常规方法检测ALT.结果 ALT异常组,大三阳HBsAg(+),HBeAg(+),Anti-HBc(+)和小三阳HBsAg(+),Anti-HBe(+),Anti-HBc(+)合并HBV DNA阳性的HBV感染者MAO显著升高,而小三阳并HBV DNA阴性者MAO升高不明显;ALT正常组仅40例大三阳患者MAO升高明显,其他各组MAO大多在正常范围内.结论 HBV感染后,只要有病毒复制,就会破坏肝细胞,引起MAO升高.MAO活性分析对肝脏病情的判断有重要价值.

Objective To analyse the activity of Monoamine Oxidase (MAO) in the patients with hepatitis B virus and to study the value of clinical application of Monoamine Oxidase.Methods The activity of Monoamine Oxidase was detected by colorimetric method.Hepatitis B virus markers were detected by ELISA.HBV DNA was detected by PCR.ALT was detected by routine technique.Results In ALT abnormal group,the level of Monoamine Oxidase were higher in the patients positive for HBsAg,HBeAg,anti-HBc and positive HBsAg,anti-HBe,anti-HBc,HBV DNA(+) than the patients positive for HBsAg,anti-HBe,anti-HBc,HBV DNA(-) (P<0.01).In ALT normal group,the level of Monoamine Oxidase were only higher in the patients positive for HBsAg,HBeAg,anti-HBc.Conclusion The level of Monoamine Oxidase will be higher as long as HBV DNA(+),so analysis of Monoamine Oxidase is very important for the judgment of liver disease.