摘要
根据国外已发表的番鸭细小病毒 (MPV) FM株基因组核苷酸序列 ,设计了 1对引物 ,分别对国内 2株番鸭细小病毒分离株 MPV扬州 (YZ)株和 MPV佛山 (FS)株主要结构蛋白 (VP2和 VP3)基因进行 PCR扩增 ,并克隆到p CR3.1T载体 ,经酶切鉴定筛选出阳性克隆质粒并进行核苷酸序列分析比较。结果表明 ,MPV YZ和 MPV FS的VP2 - VP3基因大小均为 176 4bp,编码 5 88个氨基酸。 MPV YZ、MPV FS与 MPV FM核苷酸序列同源性分别为98.5 %和 98.6 % ,氨基酸序列同源性为 97.1%和 97.8% ;MPV YZ与 MPV FS核苷酸序列的同源性为 99.5 % ,氨基酸序列同源性为 98.8%。
A pair of primers were desiged to amplify the major structural protein(VP2 and VP3) gene of MPV,according to nucleotide suquences of MPV strain FM.The VP2-VP3 genes of two domestic isolated strains(MPV FS,MPV YZ) were obtained by PCR and sequenced after being directly inserted into the pCR3.1 T vector.The sequence analysis demonstrated that the VP2-VP3 genes of two strains consisted of 1 764 bp,encoding 588 amino acids.Compared with strain FM,the homology of the nucleotide sequence are 98.6% and 98.5% the homology of the deduced amino acid sequence are 97.8% and 97.1%.These results indicated that MPV strain FS and YZ should be closely related to MPV strain FM.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2002年第3期225-227,共3页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目 ( 39870 5 5 7)
教育部和总后勤部回国人员启动基金资助项目 ( 98H0 2 6 )