番鸭细小病毒国内分离株主要结构蛋白基因的克隆和序列分析

Cloning and Sequence Analysis of Major Structural Protein(VP2 and VP3) Gene of Domestic Isolation Strains of Muscovy Duck Parvovirus

根据国外已发表的番鸭细小病毒 (MPV) FM株基因组核苷酸序列 ,设计了 1对引物 ,分别对国内 2株番鸭细小病毒分离株 MPV扬州 (YZ)株和 MPV佛山 (FS)株主要结构蛋白 (VP2和 VP3)基因进行 PCR扩增 ,并克隆到p CR3.1T载体 ,经酶切鉴定筛选出阳性克隆质粒并进行核苷酸序列分析比较。结果表明 ,MPV YZ和 MPV FS的VP2 - VP3基因大小均为 176 4bp,编码 5 88个氨基酸。 MPV YZ、MPV FS与 MPV FM核苷酸序列同源性分别为98.5 %和 98.6 % ,氨基酸序列同源性为 97.1%和 97.8% ;MPV YZ与 MPV FS核苷酸序列的同源性为 99.5 % ,氨基酸序列同源性为 98.8%。

A pair of primers were desiged to amplify the major structural protein(VP2 and VP3) gene of MPV,according to nucleotide suquences of MPV strain FM.The VP2-VP3 genes of two domestic isolated strains(MPV FS,MPV YZ) were obtained by PCR and sequenced after being directly inserted into the pCR3.1 T vector.The sequence analysis demonstrated that the VP2-VP3 genes of two strains consisted of 1 764 bp,encoding 588 amino acids.Compared with strain FM,the homology of the nucleotide sequence are 98.6% and 98.5% the homology of the deduced amino acid sequence are 97.8% and 97.1%.These results indicated that MPV strain FS and YZ should be closely related to MPV strain FM.