萱草种质资源扩增片段长度多态性鉴别与分类的研究 Ⅱ.5个萱草材料的AFLP分析

Identification and Classification of Hemerdcallis Resources with Using AFLP Molecule Marker Ⅱ.AFLP Analysis of Five Materials of Orange Daylily

为了快速准确地进行萱草种质鉴定 ,从 6 4对AFLP引物中随机选出了 3对引物组合 (即EcoRⅠAAC MseⅠCAT ,EcoRⅠACC MseⅠCAT ,EcoRⅠACC MseⅠCAG) ,构建了 5个萱草种质的指纹图谱 .3对引物在 5个萱草材料中 2 0 5个位点上扩增出了条带 ,其中多态性条带 12 8个 ,占扩增条带总数的 6 2 .9% ,5个萱草材料具有较高的遗传多样性 ,并初步认为其中野生重瓣萱草可能来自于萱草H .

In order to identify orange daylily varieties accurately and quickly, AFLP fingerprinting were obtained by using three pairs of primers EcoRⅠAAC MseⅠCAT ,EcoRⅠACC MseⅠCAT and EcoRⅠACC MseⅠCAG. Among 205 bands,there were 128 polymorphism bands.The ratio of polymorphism bands reached 62.9%.The five materials of orange daylily had higher genetic diversity,and the wild orange daylily was considered as a mutation of H.fulva.