死胎胸腺组织中HBsAg检测的临床和病理研究

Clinical and Pathologic Study on the Expression of HBsAg in the Thymus Tissues of the Died Fetusion

目的 :观察乙型肝炎病毒通过产妇传播在胎儿胸腺组织中表达的情况。方法 :采集 4 0例乙型肝炎产妇产下的死胎 ,常规尸检 ,取胸腺组织 ,SP法检测 HBs Ag;回访婴母产前静脉血 HBVM的检测结果 ;取百分率行 U检验。结果 :死胎胸腺组织胸腺小体数量明显减少 ;HBs Ag阳性颗粒在胸腺细胞间呈点状分布 ,胸腺细胞核不着色 ,毛细血管内可见到棕黄色 HBs Ag颗粒。HBV呈大三阳的婴母较 HBV呈单项阳性、小三阳的婴母分娩的死胎胸腺组织中 HBs Ag阳性率明显升高、胸腺小体数量明显减少 ,差异显著 (P<0 .0 5 ) ;HBV呈单项阳性、小三阳的婴母分娩的死胎胸腺组织中 HBs Ag阳性率、胸腺小体数量比较 ,差异不显著 (P>0 .0 5 )。结论 :婴母静脉血 HBV大三阳是乙型肝炎病毒通过母婴垂直传播在胎儿胸腺组织中表达的高危因素。

Objectives:Study the risk factors that the thymus tissue of the died fetus infected by HBV through maternal fetal transmission.Methods:40 pieces of thymus tissues of died fetus were detected by immunocytochemistry(SP) for HBsAg.Investigation were under taken for HBVM in the mother veins before dethymusy.Results:It was reduced for the amount of the thymus corpuscle;There were HBsAg in the thymus tissues.They were mainly among the thymus cells and were not in the nuclei,but they were also in the capillary vessel.The positive cells were single or form distributed over the thymus tissues.There was statistically significance( P <0.05)for the comparison between the rate of the HBcAg positive expression and the amount of the thymus corpuscle in the thymus tissues of the died fetus that the puerperal dethymused with hepatitis virus B for only one ①HBVM+,②(HBsAg+?HBeAb+?HBcAb+)and③(HBsAg+?HBeAg+?HBcAb+)in the mother veins before dethymusy.The comparison between①and② were not statistically significant( P >0.05).Conclusions:It was the risk factor for the thymus tissue of the died fetus infected by HBsAg through maternal fetal transmission that HBVM(HBsAg+?HBeAg+?HBcAb+)was with positive expresion in the mother veins before dethymusy.