慢性牙周炎患者的龈下微生物与吸烟状况

Subgingival microflora and smoking status in chronic periodontitis

目的 评价慢性牙周炎患者的吸烟状况与龈下牙周致病微生物的百分比。方法 112例慢性牙周炎患者 ,根据吸烟状况分为 :①重度吸烟组 >10支 /天 (n =32 ) ;②轻度吸烟组≤ 10支 /天 (n =18) ;③戒烟者组 (n =2 4 ) ;④非吸烟组 (n =38)。观察者口内每个象限 ,选取探诊最深的 1或 2个位点 ,纸捻法取龈下菌斑 ,厌氧培养 ;并测量该位点的探诊深度 (ProbeDepth ,PD)、附着丧失 (AttachmentLoss,AL)和探诊后出血 (Bleedingofprobe ,BOP)。结果 ①取样位点临床指标的均值为PD :6 .3mm、AL :6 .5mm及BOP :89% ,各组间无显著性差异。②组间菌落形成单位和龈下微生物伴放线放线杆菌 (Actinobacillusactinomycetemcomitans,A .a)、牙龈卟啉单胞菌 (Porphyromanasgingivalis,P .g)、中间普氏菌 (Prevotellaintermedia ,P .i)、核梭杆菌 (Fusobacteriumnucleatum ,F .n)和微消化球菌(Peptostreptococcusmicros,P .m)的百分比均无差异。③方差分析显示仅轻度吸烟组福赛类杆菌 (Bacteroidesforsythus,B .f)的百分比略高于其它组 (P <0 .0 4 )。结论 慢性牙周炎患者 ,探诊深度和附着丧失相似的位点 ,吸烟组、戒烟组和非吸烟组的龈下牙周致病微生物百分比 (B .f除外 )无显著差异。

Objective To evaluate the difference in subgingival microflora of chronic periodntitis patients in four types of smoking status.Methods 112 patients with adult periodontitis.Based on smoking status,were grouped as follows:①heavy smokers>10cig/day (n=32);②light smokers ≤10 cig/day (n=18);③former smokers who had quited smoking now (n=24); (IV) non-smoker(n=38).Samples of 1-2 deepest sites per quadrant of each patient were taken for analysis of the anaerobic microflora, then probe depth (PD), attachment loss (AL) and bleeding of probe (BOP) were measured.Results ①Mean PD, AL of these sites were 6.3 mm, 6.5 mm and BOP 89%. Meanwhile, PD, AL and BOP did not show the difference between the 4 subgroups of the patients.②No significant difference was found in percen tage among Actinobacillus actinomycetemcomitans (A.a), Porphyromonas gingivalis(P.g), Prevotella intermedia(P.i),Fusobacterium nucleatum(F.n),and Peptostreptococcus micros(P.m).③B.f(P< 0.04 ) in light smokers was significantly higher than ofter the subgroups.Conclusion The results suggest that smokers and non-smokers with periodotitis have the same population of subgingival microflora except for Bf.