血管抑素抑制G 422脑胶质瘤生长的实验研究

Experimental Study of Inhibitory Effects of Angiostatin on Glioma Line G 422

目的:研究血管抑素(AS)对荷G422脑胶质细胞系小鼠的治疗作用。方法:应用MTT法观察AS对血管内皮细胞系ECV-304和人脑胶质瘤细胞系TJ-905增殖的影响;建立G 422脑胶质瘤小鼠皮下移植模型。皮下注射AS,剂量为10mg/(kg·d)和50mg/(kg·d),连续治疗14天,观察瘤体比和抑瘤率变化。结果:AS对ECV-304细胞抑制作用呈量效依赖性关系,IC(50)=3.7mg/L。体内实验显示,50mg/(kg·d)剂量组抑瘤率达84.3％。结论:本研究为AS用于脑胶质瘤的治疗提供了实验依据,说明AS在肿瘤治疗中有广泛的应用前景。

Objective: To study the inhibitory effects of angiostatin (AS) on glioma line G422. Methods: The effects of AS on the proliferation of angioendothelial cell line ECV-304 and human glioma cell line TJ-905 were observed with MTT method. The mouse model was established for subcutaneous transplantation of glioma line G422. After injection of AS [10 and 50mg/(kg·d)] for 14 days, the changes of tumor ratio and tumor inhibition rate were observed. Results: The AS inhibitionon KCV-304 cell exhibited a dose-action dependent relation with an IC (50) to be 3.7 mg/L. The inhibition rate of 50 mg/(kg·d) in vivo might be as high as 84.3% . Conclusion. The experimental study indicates that the AS will be of a wide application in tumor treatment.