失神经支配红白肌感觉神经营养活性研究

STUDY ON SENSE NEUROTROPHISM OF DENERVATED RED AND WHITE MUSCLES

目的 探讨对失神经支配红、白肌肉内神经生长因子 (NGF)变化规律及 NGF含量与感觉神经营养活性的关系。方法 剪断大鼠坐骨神经制成腓肠肌和比目鱼肌失神经支配模型 ,分别于神经损伤后 1、3、7和 14天取材 ,运用免疫组织化学方法检测失神经支配红、白肌肉内 NGF含量 ,同时用鸡胚背根神经节培养方法测定失神经支配肌肉提取液感觉神经营养活性。结果 腓肠肌和比目鱼肌失神经支配后其 NGF含量均下降 ,两种肌肉组比较无统计学意义(P>0 .0 5 ) ,组内比较以比目鱼肌组下降明显 ;但两种失神经支配肌肉提取液感觉神经营养活性未降低 ,在伤后 7天时反高于对照组 ,两种肌肉组间比较无差异。结论 周围神经损伤会引起其相应支配靶器官 -肌肉组织内 NGF的含量发生变化 ,且具有时间性 ,但红、白肌肉内 NGF含量的变化不一致 ;失神经支配肌肉的神经营养活性是各种营养因子的共同作用结果 。

Objective To clarify the character of nerve growth factor (NGF) in the denervated red and white muscles and the relationship between the amount of NGF and sensitive neurotrophism of denervated red and white muscles. Methods The model of the denervated gastrocnemius and soleus was made by clipping the sciatic nerve of Wister rats. The immunohistochemistry was taken to measure the amount of NGF in muscles, and the neurotrophism of extracts of muscles was tested with culture of dorsal root ganglions at the 1st, 3rd, 7th and 14th days after injury. Results The amount of NGF in denervated gastrocnemius and soleus decreased, especially in soleus. The neruotrophism of the extracts of the two kinds of denervated muscles did not decrease; on the contrary, it increased after a week after injury. Conclusion The injury of peripheral nerves causes the amount of NGF in the target tissues to increase, but the change is different between the denervated muscles; the neurotrophism of the extracts of musclesis determined by all kinds of neurotrophic factors, and can not be explained by a single factor.