周围神经损伤后影响白细胞介素-1受体表达的研究

EFFECT OF INTERLEUKIN-1 ON EXPRESSION OF INTERLEUKIN-1 RECEPTOR IN KM MICE SCIATIC NERVES BEFORE AND AFTER INJURIES

目的 探讨周围神经损伤后有无白细胞介素 - 1(IL - 1)受体表达、其表达和创伤及 IL - 1干预后的变化规律。方法 选用昆明小鼠 72只制作单侧坐骨神经切割伤一期端端缝合模型 ,实验组和对照组各 36只 ,实验组损伤神经局部用 IL - 1浸泡 ,对照组用等量保存液。分别在术后 3小时 ,1、3、7、14和 2 8天用 L SAB法 ,检测神经近断端的 IL - 1受体表达 ,同时检测正常神经的 IL - 1受体表达水平。结果 正常神经有 IL - 1受体表达 ,定位于雪旺细胞膜。周围神经损伤后 ,对照组 IL - 1受体表达呈双相增强的特点 ;而实验组在 IL - 1处理后 ,其表达的双相规律不变 ,增强强度减小。结论 雪旺细胞是 IL -

Objective To study the expression of interleukin 1 receptor in normal mice sciatic nerves and in transected sciatic nerves treated with or without extrinsic interleukin 1 locally at different periods. Methods Seventy two KM mice were equally divided into two groups. All the left sciatic nerves were transected. The stumps in experimental group were soaked in liquid with interleukin 1, whereas those in control group without interleukin 1. Then all the stumps were repaired end to end. At the 3rd hour, 1st day, 3rd day, 7th day, 14th day, and 28th day after operation respectively, every proximal stump was dissected and the expression of interleukin 1 receptor was carried out by immunohistochemistry method (LSAB method). The expression level of interleukin 1 of ten normal sciatic nerves of mice was studied, too. Results Normal nerves showed interleukin 1 receptor expression on the membrane of Schwann cells. After nerve injury, the interleukin 1 receptor expression increased biphasically in both groups, but the intensity of increase was lower in the experimental group than in the control group.Conclusion Schwann cell is the target cell of interleukin 1