煤焦沥青烟对小鼠的免疫毒性研究

The Study on Immunotoxicity of Coal Tar Pitch Fumes in Mice

给小鼠每日皮下注射2.5、5.0和10.0μg/g的煤焦沥青烟,连续染毒10天,小鼠体重增长呈现不同程度的降低,胸腺和脾脏萎缩,外周血T淋巴细胞百分数及足跖迟发型变态反应明显降低。高剂量沥青烟可使脾抗体生成反应及血清抗体滴度降低,抑制率达69.5%。但未见对单核巨噬细胞碳粒廓清作用及肝、肾重量有明显影响。沥青烟对机体具有明显的细胞和体液免疫毒性。

Mice were exposed to coal tar pitch fumes ( CTPF ) at dosage of 25, 50 and 100μg/g by subcutaneous route daily for 10 days. The control mice were given DMSO alone ( DMSO ) . There was a reduction in body weight increase, thymus and spleen weights, and the peripheral T cells count at dosage of 59 and 100μg/g, and futher, the spleen antibody producing response and the serum antibody titeroof mice immunized with thymus-dependent antigen SRBC were decreased 69.5% at dosage of 100μg/g as compared to the control. In the pedal delayed allergy test the delayed allergy response was significantly suppressed at three doses. There was no differences between exposed and control group in the peripheral monophagocyte carbon granular clearance assay. It suggested that CTPF cna produce adverse effect on the humoral and cellular immunity in mice.