体外培养猴眼小梁细胞及睫状肌细胞中组织金属蛋白酶抑制剂的表达

Expression of tissue inhibitors of metalloproteinase in monkey trabecular meshwork cells and ciliary muscle cells

目的 观察体外培养的正常猴眼小梁细胞及睫状肌细胞中组织金属蛋白酶抑制剂(tissueinhibitorsofmetalloproteinase ,TIMP)的表达 ,探讨生理状态下基质金属蛋白酶 (matrixmetalloproteinase,MMP)及TIMP在小梁网房水流出及葡萄膜 巩膜房水流出通道中的作用。方法 采用Reverse zymography技术 ,检测猴眼小梁细胞及睫状肌细胞中TIMP及MMP的表达。结果 猴眼小梁细胞及睫状肌细胞培养液中均可见TIMP 1、TIMP 2及TIMP 3表达 ,小梁细胞中MMP/TIMP比值明显高于睫状肌细胞。结论 正常状态下小梁细胞外基质的降解能力明显高于睫状肌细胞 ,可能是由于传统的小梁网房水流出通道作用强于葡萄膜 巩膜房水流出通道的作用。

Objective To identify tissue inhibitors of metalloproteinase (TIMP) released by normal monkey trabecular meshwork cells and ciliary muscle cells in vitro and to detect the roles of matrix metalloproteinase (MMP) and TIMP playing in trabecular outflow and uveoscleral outflow in normal eyes Methods The expressions of TIMP and MMP in monkey trabecular meshwork and ciliary muscle cultures were detected by the technology of reverse zymography Results (1) TIMP 1, TIMP 2 and TIMP 3 were found in both trabecular meshwork and ciliary muscle cultured cells (2) The ratio of MMP/TIMP in trabecular meshwork cells was much higher than that in ciliary muscle cells Conclusions The different ratio of MMP/TIMP in these two types of cells suggests that the capability of degrading extracellular matrix in trabecular meshwork cells be much higher than that in ciliary muscle cells, that may be one of the reasons why conventional trabecular outflow is stronger than uveoscleral outflow