地塞米松诱导牛眼小梁细胞凋亡的研究

Apoptosis of bovine trabecular meshwork cells induced by dexamethasone

目的 探讨地塞米松诱导牛眼小梁细胞凋亡的机制。方法 采取体外组织块培养法 ,培养新生牛眼小梁细胞。将 1～ 5 0 0mg/L地塞米松加入融合的第 3～ 5代小梁细胞培养液中 ,作用1～ 14d ,用相差显微镜、荧光显微镜、透射电子显微镜、DNA电泳及流式细胞仪检测细胞凋亡情况。结果 12 5～ 5 0 0mg/L地塞米松作用 1～ 14d ,小梁细胞凋亡呈浓度时间依赖性。凋亡的小梁细胞经Hoechst 332 5 8染色 ,于荧光显微镜下观察 ,细胞核呈致密浓染的颗粒块状荧光 ;透射电镜下可见小梁细胞染色质固缩 ,并有凋亡小体 ,细胞器基本完好 ;DNA电泳呈梯状条带 ;流式细胞仪检测到亚二倍体峰。结论 地塞米松可诱导体外培养的小梁细胞凋亡 ,可能是激素性青光眼的发病机制之一。

Objective To investigate apoptosis of bovine trabecular meshwork cells induced by dexamethasone Methods In the incubation method of system in vitro, dexamethasone (1 500 mg/L) was added into the syncretic culture solution of the third to fifth generation of bovine trabecular meshwork cells After 1 14 days, the culture solution was observed by phase contrast microscopy, fluorescence microscopy and transmission electron microscopy, and was studied by DNA laddering and flow cytometric analysis Results Dexamethasone (125 500 mg/L) induced apoptosis of trabecular meshwork cells in a dose time dependent manner Conclusions Apoptosis of cultured bovine trabecular meshwork cells can be induced by dexamethasone, which may be one of the pathogenic mechanisms of steroid induced glaucoma