维生素A和3-甲基胆蒽体外致畸作用及其与GSH-GST变化的关系

The Effects of Vit A and 3-MCA on GSH-GST in Teratogenicity in vitro

用妊娠第14天大鼠胚胎中脑神经细胞作微团培养,培养物接触维生素A(VitA)和3-甲基胆蒽(3-MCA)后,微团中的集落形成率明显降低,半数分化抑制浓度分别为10ng/ml和1.0ng/ml,有明显的致畸性。在培养12小时内,还原型谷胱甘肽(GSH)和谷胱甘肽S-转移酶(GST)水平明显降低,24小时后逐步快复正常,而GST活性继续升高,于48小时达最大值。这两种化学物的体外致畸作用可能与GSH耗竭有一定关系。

The teratogenicity of vit A and 3-MCA was studied in a micromass culture system in vitro. The teratogenicity was expressed as decrement of counts of differentiated foci. The differentiating rat embryo midbrain cells exposing to nontoxic doses of vit A or 3-MCA showed inhibition on subsequent differentiation. It was observed that glutathione was depleted and glutathione S-transferase was inhibited by vit A or 3-MCA in the cultures at 12h, and then both were reversed and increased after 24h. The concentration-response relationship of the varieties was apparent. The results in present study indicated the possibility that the varieties of glutathione system would affect the subsequent differentiation of midbrain cells.