1,3-二氯苯降解细菌Bacillus cereus PF-11的遗传选育及其降解特性研究

Effective biodegradation of 1,3-dichlorobenzene by a mutant of Bacillus cereus PF-11

以焦化厂废水中分离得到的一些苯降解细菌为出发菌 ,通过遗传突变方法获得了一株高效降解 1,3 二氯苯的突变株PF 11.在合适条件下突变株PF 11培养液对 1,3 二氯苯的降解速率可达 31mg (L·d) ,并伴随着有效的氯离子释放 .经初步鉴定 ,突变株PF 11为以前未见报导的革兰氏阳性Bacilluscercus菌 .菌株PF 11的底物专一性较低 ,对单氯苯和 1,2 二氯苯均有降解作用 ,降解能力为单氯苯 >1,3 二氯苯 >1,2 二氯苯 .

an effective 1,3 dichlorobenzene degrading strain PF 11 from benzene utilizing bacteria isolated from town gas industrial effluent by genetic breeding was introduced. A degradation rate of 31mg/(L·d) of 1,3 DCB could be obtained by strain PF 11 in suitable condition with effective chlorion release. Mutant PF 11 was tentatively identified as gram positive Bacillus cereus , which has not been reported in detail. The substrate specificity of mutant PF 11 was relatively low, and the degradation ability to different chlorobenzene was in the order of MCB> 1,3DCB >1,2DCB with initial attacking by molecular oxygen catalysed by dioxygenase.