大气PM_(2.5)对巨噬细胞的毒性作用研究

Study on Toxic Effect of Atmospheric PM_(2.5) on Macrophages

以上海市工业区、交通区、郊区3个功能区的大气PM2.5粒子,对腹腔巨噬细胞进行染毒培养,台酚蓝染色测定细胞存活率,酵母菌吞噬实验测定其吞噬功能,流式细胞仪检测凋亡情况。结果显示,随大气PM2.5粒子浓度增加,巨噬细胞存活率和吞噬功能下降,巨噬细胞出现凋亡,其中交通区大气PM2.5染毒组的观察指标改变较明显。提示了大气PM2.5粒子对巨噬细胞具有细胞毒性,而交通区大气PM2.5粒子的毒性较大。

PM2.5 particles samples have been collected from three different functional zones in Shanghai, namely: industrial area, traffic area and suburb. After administered for 24 hours, the cells survival rate of peritoneal macrophages were measured by trypan blue dyeing, phagocytic function of macrophages was determined by yeast phagocytosis method, and apoptosis was inspected by flow cytometer. The results were as follows: With increase of concentration of atmospheric PM2.5 particles, the survival rate and phagocytic function of peritoneal macrophages decreased, apoptosis of macrophages was found to have a dose-dependent increase. In addition, atmospheric PM2.5 particles from traffic area caused more obvious alteration in three indices than those from other areas. So the conclusion could be drawn that atmospheric PM2.3 particles have obvious immune toxicity on macrophages and the immune toxicity of atmospheric PM2.5 particles from traffic area was stronger than those from the industrial area and suburb.