反相高效液相色谱法测定痕量金属离子的研究

Determination of Trace Metal Ions by RP-HPLC

本文研究了无机材料常见的金属离子铜(Ⅱ)、镍(Ⅲ)、钴(Ⅱ)与 DDTC (二乙基二硫氨基甲酸钠)形成的螯合体系在反相高效液相色谱(RP—HPLC)柱上的保留行为,得到了金属离子铜(Ⅱ)、镍(Ⅱ)、钴(Ⅱ)在色谱柱上分离的最优条件,流动相的最优组成是甲醇:0.02mol/L NaH_2PO_4=88∶12(V/V),流速0.8mL/min,检测器波长330nm 和270nm。并对柱内与柱外不同络合方式进行初步探索。

In this paper,the retentive behavior of metal ions Cu(Ⅱ),Co(Ⅱ) and Ni(Ⅱ)in inorganic material chelating complexes of DDTC on column of reversed phasehigh-performence liquid chromatography (RP-HPLC) has been investigated,andoptimum conditions of separating metal ions Cu(Ⅱ),Co(Ⅱ) and Ni(Ⅱ) fromchromatographic column decided.The optimum composition of the mobile phase isMeOH:0.02 mol/L NaH_2PO_4=88:12(V/V).the mobile velocity is 0.8 ml/min,wavelengths used for measurement are 330 nm and 270 nm.Both pre-column andinsitu different methods have been investigated.